University of Pennsylvania
Department of Pathology and Laboratory Medicine
Division of Transfusion Medicine
Research Publications, peer reviewed
1. Heindel, N.D., Vandongen, J.M.A.M., Sachais, B.S., Phillips, J.H., Gallo, M.A. and Laskin, J.D. Synthesis of psoralen analogues and evaluation of their inhibition of epidermal growth factor binding. J. Pharmacol. Sci. Vol. 80: 686-689, 1991.
2. Takeda, Y., Chou, K.B., Takeda, J., Sachais, B.S. and Krause , J.E. : Molecular cloning, structural characterization and functional expression of the human substance P receptor. Biochem. Biophys. Res. Comm. Vol. 179: 1232-1240, 1991.
3. Takeda, Y., Blount, P., Sachais, B.S., Hershey, A.D., Raddatz, R. and Krause, J.E. : Ligand binding kinetics of substance P and neurokinin A receptors stably expressed in Chinese hamster ovary cells and evidence for differential stimulation of inositol 1,4,5-trisphosphate and cyclic AMP second messenger responses. J. Neurochem. Vol. 59: 740-745, 1992.
4. Krause, J.E., Bu, J.-Y., Takeda, Y.,Blount, P., Raddatz, R., Sachais, B.S., Chou, K.B., Takeda, J., McCarson, K. and DiMaggio, D. : Structure, expression, and second messenger-mediated regulation of the human and rat substance P receptors and their genes. Regul. Peptides Vol. 46: 56-66, 1993.
5. Sachais, B.S., Snider, R.M., Lowe, J.A. III, and Krause, J.E. : Molecular basis for the species selectivity of the substance P antagonist CP-96,345. J. Biol. Chem. Vol. 268: 2319-2323, 1993.
6. Sachais, B.S., and Krause, J.E. : Both extracellular and transmembrane residues contribute to the species selectivity of the neurokinin-1 receptor antagonist WIN 51708. Mol. Pharmacol. Vol. 46: 122-128, 1994.
7. Brodbeck, R.M., Sachais, B.S., and Krause, J.E. : Residue 78 in the second transmembrane domain of the neurokinin-1 receptor is important in coupling high affinity agonist binding to multiple second messenger responses. Mol. Pharmacol. Vol. 47: 1065-1071, 1995.
8. Li, H., Hsu, P., Sachais, B.S., Krause, J.E., Leeman, S.E., and Boyd, N.D. : Identification of the site in the substance P (NK-1) receptor for modulation of peptide binding by sulfhydryl reagents. J. Biol. Chem. Vol. 271: 1950-1956, 1996.
9. Sachais, B.S., Nachamkin, I. , Mills J.K., and Leonard, D.G.B. : Novel pncA mutations in pyrazinamide-resistant isolates of mycobacterium tuberculosis. Molecular Diagnosis Vol. 3: 229-232, 1998.
10. Bdeir, K., Cane, W., Canziani, G. Chaiken, I., Weisel, J., Koschinsky, M.L., Lawn, R.M., Bannerman, P.G., Sachais, B.S., Kuo, A., Hancock, M.A., Tomaszewski, J., Raguhanath, P. N., Ganz, T., Higazi, A. A-R. and Cines, D.B.: Defensin promotes the binding of Lp(a) to vascular matrix. Blood Vol. 94: 2007-2019, 1999.
11. Bdeir, K., Kuo, A., Mazar, A., Sachais, B.S., Xiao, W., Gawlak, S., Harris, S., and Higazi, A. A.-R., and Cines, D.B.: A region in domain II or the urokinase receptor required for urokinase binding. J. Biol. Chem. Vol. 275: 28532-28538, 2000.
12. Guo, Y., Higazi, A. A-R., Arkelian, A., Sachais, B.S., Cines, D.B., Goldfarb, R.H., Jones, T.R., Kwaan, H., Mazar, A. P., and Rabbani, S.A. : A peptide derived from the urokinase plasminogen activator (uPA) inhibits tumor progression and angiogenesis and induces tumor cell death in vivo. FASEB J. Vol. 14: 1400-1410, 2000.
13. Haj-Yehia,A., Nasser, T., Sachais, B.S., Kuo, A., Bdeir, K., Al-Mehdi, A.B., Mazar, A.P., Cines, D.B., and Higazi, A. A-R.: Urokinase-derived peptides regulate vascular smooth muscle contraction in vitro and in vivo. FASEB J. Vol. 14: 1411-1422, 2000.
14. Higazi, A. A.-R., Nassar, T., Ganz, T.,Rader, D., Udassin, R., Bdeir, K.,Hiss, R., Sachais, B.S., Williams,K.J., Leitersdorf, E. and Cines, D.B. The a-defensins stimulate proteoglycan-dependent catabolism of low-density lipoprotein by vascular cells: A new class of inflammatory apolipoprotein and a possible contributor to atherogenesis Blood Vol. 96: 1293-1398, 2000.
15. Shliom, O., Huang, M., Sachais, B.S., Kuo, A., Weisel, J.W., Nagaswami, C., Nassar, T., Bdeir, K., Hiss, E., Gawlak, S., Harris, S., Mazar, A., and Higazi, A. A.-R.: Novel interactions between urokinase and its receptor. J. Biol. Chem. Vol. 275: 24304-24312, 2000.
16. Young, D.S., Sachais, B.S. and Jefferies, L.C.: Laboratory costs in the context of disease Clin Chem Vol. 46: 976-988, 2000.
17. Young, D.S., Sachais, B.S. and Jefferies, L.C.: The costs of disease Clin Chem Vol. 46: 955-966, 2000.
18. Jefferies, L.C., Sachais, B.S. and Young, D.S. : Blood transfusion costs by diagnosis-related groups in 60 university hospitals in 1995 Transfusion Vol. 41: 522-529, 2001.
19. MacDonald, D., Mierke, D.F., Li, H., Pellegrini, M., Sachais, B., Krause, J.E., Leeman, S.E., and Boyd , N.D. : Photoaffinity labeling of mutant neurokinin-1 receptors reveals additional structural features of the substance P/NK-1 receptor complex. Biochemistry Vol. 40: 2530-2539, 2001.
20. Reilly, M.P., Taylor , S.M., Hartman, N.K., Arepally, G.M., Sachais, B.S., Cines, D.B., Poncz, M., McKenzie, S.E: Heparin-induced thrombocytopenia/thrombosis in a transgenic mouse model requires human platelet factor 4 and platelet activation through FcgRIIA. Blood Vol. 98: 2442-2447 2001.
21. Zhang, C., Thornton , M.A., Kowalska, M.A., Sachais, B.S., Feldman, M., Poncz, M., McKenzie, S.E., and Reilly, M.P.: Localization of distal regulatory domains in the megakaryocyte-specific Platelet Basic Protein (PBP)/Platelet Factor 4 (PF4) gene locus. Blood Vol. 98: 610-617, 2001.
22. Sachais, B.S., Kuo, A., Nassar, T., Morgan, J., Kariko, K., Williams, K.J., Feldman, M., Aviram, M. , Shah, N., Jarett, L., Poncz, M., Cines, D.B., and Higazi, A.-A.R: Platelet factor 4 binds to low-density lipoprotein receptors and disrupts the endocytic itinerary, resulting in retention of low-density lipoprotein on the cell surface. Blood Vol. 99: 3613-3622, 2002.
23. Sachais, B.S., Thompson, J. and Strobl, F.: Use of plasma exchange and steroids is a case of multiple arterial thromboses in a patient with IgM anti-cardiolipin antibodies. Journal of Clinical Apheresis 17(3): 138-139, 2002.
24. Young, D.S., Sachais, B.S., and Jefferies, L.C. : Effect of disease complications on hospital costs Clin. Chem. Vol. 48: 140-149, 2002.
25. Young, D.S., Sachais, B.S., and Jefferies, L.C. : Comparative costs of treating adults and children within selected diagnosis-related groups. Clin. Chem. Vol. 48: 150-160, 2002.
26. Zhang, C., Liu, K., Park, K.S., Sachais, B.S., Arepally, G.M., Cines, D.B., and Poncz, M. : Defining a second epitope for heparin-induced thrombocytopenia/thrombosis antibodies using KKO, a murine HIT-like monoclonal antibody. Blood Vol. 99: 1230-1236, 2002.
27. Kufrin, D., Eslin, D.E., Bdeir, K., Murciano, J.C., Kuo A., Kowalska, M.A., Degen, J.L., Sachais, B.S., Cines ,D.B., and Poncz, M.: Antithrombotic thrombocytes: ectopic expression of urokinase-type plasminogen activator in platelets. Blood 102(3): 926-33, August 2003.
28. Bdeir, K., Kuo, A., Sachais, B.S., Rux, A.H., Bdeir, Y., Mazar, A., Higazi, A.A,. and Cines, D.B.: The kringle stabilizes urokinase binding to the urokinase receptor. Blood 102(10): 3600-3608, 2003.
29. Pitsilos, S., Hunt, J.L., Mohler, E.R., 3rd, Prabhakar, A.M., Poncz, M., Dawicki, J. , Khalapyan, T., Wolfe, M., Fairman, R., Mitchell, M., Carpnter, J., Golden, M.A., Cines, D.B., Sachais, B.S.: Platelet factor 4 localization in carotid atherosclerotic plaques: Correlation with clinical parameters. Thrombosis and Haemostasis Vol. 90: 1142-1150, 2003.
30. Sachais, B.S., Nassar, T., Akkawi, S., Kowalska, M.A., Bdeir, K., Leitersdorf, E., Hiss, E., Ziporen, L., Aviram, M., Cines, D., Poncz, M., Higazi, A.A-R : Platelet factor 4 enhances the binding of oxidized low-density lipoprotein to vascular wall cells. J Biol Chem. Vol. 278: 6187-6193, 2003.
31. Yarovoi H., Kufrin D., Eslin D.E., Thornton M.A., Haberichter S.L., Shi Q., Zhu H., Camire R., Fakharzadeh S.S., Kowalska M.A., Wilcox D.A., Sachais B.S., Montgomery R.R., Poncz M.: Factor VIII ectopically expressed in platelets: efficacy in hemophilia A treatment. Blood. 102(12): 4006-4013, December 2003.
32. Rauova, L., Poncz, M., McKenzie, S.E., Reilly, M.P., Arepally, G., Weisel, J.W., Nagaswami, C., Cines, D.B., and Sachais, B.S.: Ultralarge Complexes of Heparin and PF4 are Central to the Pathogenesis of Heparin-Induced Thrombocytopenia. Blood Vol. 105: 131 - 138, January 2005.
33. Yu G., Rux A.H., Ma P., Bdeir K., and Sachais B.S: Endothelial expression of E-selectin is induced by the platelet specific chemokine Platelet Factor 4 through LRP in an NF- {kappaB dependent manner. BLOOD Vol. 105: 3545-3551, May 2005.
34. Suvarna, S., Rauova, L, McCracken, E.K., Goss, C.M., Sachais, B.S., McKenzie, S.E., Reilly, M.P., Gunn, M.D., Cines, D.B., Poncz, M., Arepally,G.: PF4/Heparin complexes are T-cell dependent antigens. Blood 106(3): 929-931, August 2005.
35. Sachais, B.S., Katz, J., Ross, J., and Rader, D.J. : Long-term effects of LDL apheresis in patients with severe hypercholesterolemia. Journal of Clinical Apheresis 20(4): 252-255, December 2005.
36. Reilly, M. P., Taylor , S. M., Franklin , C., Sachais, B.S., Cines, D.B., Williams, K.J., and Mckenzie, S.E.: Prothrombotic factors enhance heparin-induced thrombocytopenia and thrombosis in vivo in a mouse model Journal of Thrombosis and Haemostasis Vol. 4: 2687-2694, December 2006.
37. Cines D.B., Rauova, L., Arepally, G., Reilly, M.P., McKenzie, S.E., Sachais, B.S. and Poncz, M.: Heparin-Induced Thrombocytopenia: An Autoimmune Disorder Regulated Through Dynamic AutoantigenAssembly/Disassembly. Journal of Clinical Apheresis Vol. 22: 31 - 36, 2007.
38. Sachais, B.S., Turrentine, T., Dawicki-McKenna, J.M., Rux, A.H., Rader, D. and Kowalska, M.A. : Elimination of Platelet Factor 4 (PF4) from Platelets Reduces Atherosclerosis in C57Bl/6 and apoE-/- Mice. Thrombosis and Haemostasis Vol. 98: 1108-1113, 2007.
39. Zhang, P., Pan, W., Rux, A.H., Sachais, B.S., and Zheng, X.L.: The cooperative activity between the carboxyl-terminal TSP-1 repeats and the CUB domains of ADAMTS13 is crucial for recognition of von Willebrand factor under flow. BLOOD Vol. 110: 1887-1894, 2007.
Research Publications, peer-reviewed reviews
1. Grau, Jillian R., and Sachais, B.S.: LDL Apheresis for Patients with Severe Hypercholesterolemia. Check Sample: Transfusion Medicine NO. TM 05-8 (TM-284). American Society for Clinical Pathology, Page: 93-107, October 2005.
2. Lambert, M.P., Sachais, B.S., and Kowalska, M.A. : Chemokines and thrombogenicity. Thrombosis and Haemostasis 97(5): 722-9, April 2007.
Editorials, Reviews, Chapters
1. Krause, J.E., Sachais, B.S. and Blount, P. : Tachykinin receptors. In: Handbook of receptors and channels , edited by Peroutka, S.J. Boca Raton: CRC Press Page: 277-298, 1993.
2. Krause, J.E., Blount, P. and Sachais, B.S.: Molecular biology of mammalian tachykinin receptors: Structure, expression and regulatory mechanisms In: Tachykinin Receptors , Totowa , NJ : Humana Press 1994.
3. Sachais, B.S.: Bacterial contamination of blood products In: Handbook of Transfusion Medicine. Editors: C.D. Hillyer, K.L. Hillyer, F.J. Strobl, L.C. Jefferies, and L.E. Silberstein , New York : Academic Press 2001.
4. Sachais, B.S.: Platelet-endothelial interactions in atherosclerosis. Curr Atheroscler Rep, 3, 412 2001.
5. Sachais, B.S., Al-Roof Higazi, A., Cines, D.B., Poncz, M., Kowalska, M.A.: Interaction of platelet factor 4 with the vessel wall. Seminars in Thrombosis and Hemostasis 30(3): 351-358, June 2004.
6. Sachais, B.S.: Minibodies to Maximize Platelets? Blood Vol. 105: 434-5, January 2005.
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