The purpose is to estimate the number of cells present in the surface
of the culture by photographing them, without disturbing the culture.
Assumptions: All cells are similar in size. Algorithm: (1) Convert the image to a binary image; (2) build the histogram function of
number of regions (vertical) vs. region area (horizontal;) (3) find the widest horizontally that has the lowest
slope; (4) find the representative cell in terms of size; (4) find the number of cells in each region;
and (5) finally the total number of cells. Detaled algorithm and results can be found in our reference [51].