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Estimation of cell counts from cell culture images

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The purpose is to estimate the number of cells present in the surface of the culture by photographing them, without disturbing the culture.
 
Assumptions: All cells are similar in size. Algorithm: (1) Convert the image to a binary image; (2) build the histogram function of number of regions (vertical) vs. region area (horizontal;) (3) find the widest horizontally  that has the lowest slope; (4)  find the representative cell in terms of size; (4) find the number of cells in each region; and (5) finally the total number of cells. Detaled algorithm and results can be found in our reference [51].

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